Journal: eLife

Article Title: Persistent epigenetic memory impedes rescue of the telomeric phenotype in human ICF iPSCs following DNMT3B correction

doi: 10.7554/eLife.47859

Figure Lengend Snippet:

Article Snippet: To determine enrichment of DNMT3B binding, chromatin from iPSCs was immunoprecipitated with 5 μg of anti-DNMT3B (Diagenode Cat # C15410218) using Magna ChIP (17–610, MERCK). qPCR was performed on the immunoprecipitated samples using primers described in .

Techniques: Western Blot, Recombinant, Plasmid Preparation, CRISPR, Clone Assay, Sequencing, Protease Inhibitor, Purification, Reverse Transcription, Modification, DNA Amplification, Staining, SYBR Green Assay, Software

Journal: Cancer Science

Article Title: RAF may induce cell proliferation through hypermethylation of tumor suppressor gene promoter in gastric epithelial cells

doi: 10.1111/j.1349-7006.2008.01017.x

Figure Lengend Snippet: The expressions of Dnmt1 and Dnmt3b in mRNA or protein levels were upregulated in constitutively active RAF (caRAF)‐GES‐1. PD98059 reversed the upregulation of Dnmt1. Total RNA and protein from GES‐1 expressing caRAF or vector only, along with caRAF‐GES‐1 cells treated with 25 µM PD98059. (a) The graphical representation of the Dnmt1, Dnmt3a and Dnmt3b mRNA, data are mean ± standard deviation of triplicates of a representative experiment. Similar results were obtained in two experiments. (b) Cell lysates were used for analyzed by western blot with antibody specific for Dnmt1, Dnmt3a and Dnmt3b, β‐actin serving as a loading control. (c) caRAF can increase the activity of DNA methyltransferase in GES‐1 (216 ± 13%) and AGS (193 ± 32%) cell lines compared with each control. DMSO, dimethylsulfoxide.

Article Snippet: The following antibodies were used: MEK1/2, ERK1/2, phospho‐B‐RAF (Ser445), phospho‐MEK1/2, phosphor‐ERK1/2 (Cell Signaling Technology); Dnmt1, Dnmt3a, Dnmt3b (BD Biosciences); β‐actin (Sigma Chemical); cyclin D1, p27 KIP1 p16 INK4A (Santa Cruz Biotechnology, Santa Cruz, CA, USA); or p21 WAF1 (Calbiochem, Germany).

Techniques: Expressing, Plasmid Preparation, Standard Deviation, Western Blot, Activity Assay

Journal: Cancer Science

Article Title: RAF may induce cell proliferation through hypermethylation of tumor suppressor gene promoter in gastric epithelial cells

doi: 10.1111/j.1349-7006.2008.01017.x

Figure Lengend Snippet: Sequences of primers and probes for real‐time polymerase chain reaction

Article Snippet: The following antibodies were used: MEK1/2, ERK1/2, phospho‐B‐RAF (Ser445), phospho‐MEK1/2, phosphor‐ERK1/2 (Cell Signaling Technology); Dnmt1, Dnmt3a, Dnmt3b (BD Biosciences); β‐actin (Sigma Chemical); cyclin D1, p27 KIP1 p16 INK4A (Santa Cruz Biotechnology, Santa Cruz, CA, USA); or p21 WAF1 (Calbiochem, Germany).

Techniques: